MicroRNA-195a-5p Regulates Blood Pressure by Inhibiting NKCC2A.

ABSTRACT:

Background

Previous studies showed that miR-195a-5p was among the most abundant microRNAs (miRNAs) expressed in the kidney.

Methods

Lentivirus silencing of tumor necrosis factor-α (TNF) was performed in vivo and in vitro. Luciferase reporter assays confirmed that bumetanide-sensitive Na⁺-K⁺-2Cl^- cotransporter isoform A (NKCC2A) mRNA is targeted and repressed by miR-195a-5p. Radiotelemetry was used to measure mean arterial pressure.

Results

TNF upregulates mmu-miR-195a-5p, and -203 and downregulates mmu-miR-30c and -100 in the medullary thick ascending limb of male mice. miR-195a-5p was >3-fold higher in the renal outer medulla of mice given an intrarenal injection of murine recombinant TNF, whereas silencing TNF inhibited miR-195a-5p e

SUBMITTER: Hao S 

PROVIDER: S-EPMC9852070 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature