The adaptive response of the mandibular condyle to increased load is disrupted by ADAMTS5 deficiency.

ABSTRACT:

Objective

To determine the impact of increased load on the temporomandibular joint (TMJ) from mice deficient in the extracellular matrix protease ADAMTS5.

Materials and methods

Wire springs exerting 0.5 N for 1 h/day for 5 days (Adamts5^+/+ -n = 18; Adamts5^-/- n = 19) or 0.8 N for 1 h/day for 10 days (Adamts5^+/+-n = 18; Adamts5^-/- n = 17) were used to increase murine TMJ load. Safranin O-staining was used to determine mandibular condylar cartilage (MCC) morphology. Chondrogenic factors Sox9 and aggrecan were immunolocalized. Microcomputed topography was employed to evaluate mineralized tissues, and Tartrate-Resistant Acid Phosphatase staining was used to quantify osteoclasts.

Results

Increased load

SUBMITTER: C Porto S 

PROVIDER: S-EPMC9852085 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature