TSPO expression in a Zika virus murine infection model as an imaging target for acute infection-induced neuroinflammation.

ABSTRACT:

Introduction

Zika virus (ZIKV) is a neurotropic human pathogen that causes neuroinflammation, whose hallmark is elevated translocator protein (TSPO) expression in the brain. This study investigates ZIKV-associated changes in adult brain TSPO expression, evaluates the effectiveness of TSPO radioligands in detecting TSPO expression, and identifies cells that drive brain TSPO expression in a mouse infection model.

Methods

The interferon-deficient AG129 mouse infected with ZIKV was used as neuroinflammation model. TSPO expression was evaluated by tissue immunostaining. TSPO radioligands, [³H]PK11195 and [¹⁸F]FEPPA, were used for in vitro and ex vivo detection of TSPO in infected brains. [¹⁸F]FEPPA-PET was used for in vivo detection of TSPO expres

SUBMITTER: Victorio CBL 

PROVIDER: S-EPMC9852192 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature