Scm⁶A-seq reveals single-cell landscapes of the dynamic m⁶A during oocyte maturation and early embryonic development.

ABSTRACT: N⁶-methyladenosine (m⁶A) has been demonstrated to regulate RNA metabolism and various biological processes, including gametogenesis and embryogenesis. However, the landscape and function of m⁶A at single cell resolution have not been extensively studied in mammalian oocytes or during pre-implantation. In this study, we developed a single-cell m⁶A sequencing (scm⁶A-seq) method to simultaneously profile the m⁶A methylome and transcriptome in single oocytes/blastomeres of cleavage-stage embryos. We found that m⁶A deficiency leads to aberrant RNA clearance and consequent low quality of Mettl3^Gdf9 conditional knockout (cKO) oocytes. We further revealed that m⁶A regulates the translation and stabil

SUBMITTER: Yao H 

PROVIDER: S-EPMC9852475 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature