Chemogenetic emulation of intraneuronal oxidative stress affects synaptic plasticity.

ABSTRACT: Oxidative stress, a state of disrupted redox signaling, reactive oxygen species (ROS) overproduction, and oxidative cell damage, accompanies numerous brain pathologies, including aging-related dementia and Alzheimer's disease, the most common neurodegenerative disorder of the elderly population. However, a causative role of neuronal oxidative stress in the development of aging-related cognitive decline and neurodegeneration remains elusive because of the lack of approaches for modeling isolated oxidative injury in the brain. Here, we present a chemogenetic approach based on the yeast flavoprotein d-amino acid oxidase (DAAO) for the generation of intraneuronal hydrogen peroxide (H₂O₂). To validate this chemogenetic tool, DAAO and HyPer7, an ultrasensitive genetically e

SUBMITTER: Kalinichenko AL 

PROVIDER: S-EPMC9852792 | biostudies-literature | 2023 Apr

REPOSITORIES: biostudies-literature