Secreted PGK1 and IGFBP2 contribute to the bystander effect of miR-10b gene editing in glioma.

ABSTRACT: MicroRNA-10b (miR-10b) is an essential glioma driver and one of the top candidates for targeted therapies for glioblastoma and other cancers. This unique miRNA controls glioma cell cycle and viability via an array of established conventional and unconventional mechanisms. Previously reported CRISPR-Cas9-mediated miR-10b gene editing of glioma cells in vitro and established orthotopic glioblastoma in mouse models demonstrated the efficacy of this approach and its promise for therapy development. However, therapeutic gene editing in patients' brain tumors may be hampered, among other factors, by the imperfect delivery and distribution of targeting vectors. Here, we demonstrate that miR-10b gene editing in glioma cells triggers a potent bystander effect that leads to the selective cell

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9852814 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature