ADAR RNA editing on antisense RNAs results in apparent U-to-C base changes on overlapping sense transcripts.

ABSTRACT: Despite hundreds of RNA modifications described to date, only RNA editing results in a change in the nucleotide sequence of RNA molecules compared to the genome. In mammals, two kinds of RNA editing have been described so far, adenosine to inosine (A-to-I) and cytidine to uridine (C-to-U) editing. Recent improvements in RNA sequencing technologies have led to the discovery of a continuously growing number of editing sites. These methods are powerful but not error-free, making routine validation of newly-described editing sites necessary. During one of these validations on DDX58 mRNA, along with A-to-I RNA editing sites, we encountered putative U-to-C editing. These U-to-C edits were present in several cell lines and appeared regulated in response to specific environmental stimuli. T

SUBMITTER: Pecori R 

PROVIDER: S-EPMC9852825 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature