Serum metabolic profiling of rats infected with Clonorchis sinensis using LC-MS/MS method.

ABSTRACT:

Background

Clonorchiasis is an important foodborne parasitic disease. The omics-based-techniques could illuminate parasite biology and further make innovations in the research for parasitic diseases. However, knowledge about the serum metabolic profiles and related metabolic pathways in clonorchiasis is very limited.

Methods

A untargeted ultra-high performance liquid tandem chromatography quadrupole time of flight mass spectrometry (UHPLC-QTOF-MS) was used to profile the serum metabolites of rats at both 4 and 8 weeks post infection (wpi) with Clonorchis sinensis (C. sinensis). Additionally, multivariate statistical analysis methods were employed to identify differential metabolites. Next, serum amino acids and phosphatidylcholines (PCs) levels were determined

SUBMITTER: Han S 

PROVIDER: S-EPMC9852996 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature