Proteiniphilum and Methanothrix harundinacea became dominant acetate utilizers in a methanogenic reactor operated under strong ammonia stress.

ABSTRACT: Microorganisms in anaerobic digestion (AD) are easily affected by ammonia, especially acetoclastic methanogens. Thus, in ammonia-suppressed AD systems, acetate degradation is reported to be carried out mainly by the cooperation of syntrophic acetate oxidizers and hydrogenotrophic methanogens. Previous studies have revealed ammonia inhibition on microbial flora by AD performance, but the effect mechanism of ammonia on microbial metabolism remains poorly understood. In this study, we constructed a mesophilic chemostat fed with acetate as the sole carbon source, gradually increased the total ammonia nitrogen (TAN) concentration from 1 g L^-1 to 6 g L^-1, and employed the 16S rRNA gene, metagenomics, and metatranscriptomics analysis to characterize the microbial community s

SUBMITTER: Feng G 

PROVIDER: S-EPMC9853277 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature