Real-Time Monitoring of Biofilm Formation Using a Noninvasive Impedance-Based Method.

ABSTRACT: Biofilms are complex three-dimensional microbial communities that adhere to a variety of surfaces and interact with their surroundings. Because of the dynamic nature of biofilm formation, establishing a uniform technique for quantifying and monitoring biofilm volume, shape, and features in real-time is challenging. Herein, we describe a noninvasive electrochemical impedance approach for real-time monitoring of dental plaque-derived multispecies biofilm growth on a range of substrates. A working equation relating electrochemical impedance to live biofilm volume has been developed that is applicable to all three surfaces examined, including glass, dental filling resin, and Ca²⁺-releasing resin composites. Impedance changes of 2.5, 35, 50, and 65% correlated to biofilm volumes of 0

SUBMITTER: Kumar S 

PROVIDER: S-EPMC9853957 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature