Tanshinone IIA ameliorates Aβ transendothelial transportation through SIRT1-mediated endoplasmic reticulum stress.

ABSTRACT:

Background

The disruption of blood-brain barrier (BBB), predominantly made up by brain microvascular endothelial cells (BMECs), is one of the characteristics of Alzheimer's disease (AD). Thus, improving BMEC function may be beneficial for AD treatment. Tanshinone IIA (Tan IIA) has been proved to ameliorate the cognitive dysfunction of AD. Herein, we explored how Tan IIA affected the function of BMECs in AD.

Methods

Aβ_1-42-treated brain-derived endothelium cells.3 (bEnd.3 cells) was employed for in vitro experiments. And we performed molecular docking and qPCR to determine the targeting molecule of Tan IIA on Sirtuins family. The APP^swe/PS^dE9 (APP/PS1) mice were applied to perform the in vivo experiments. Following the behavioral tests, protei

SUBMITTER: Wan C 

PROVIDER: S-EPMC9854034 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature