Implementing a novel capture and ligation probe-PCR method in mass screen and treatment to support malaria elimination efforts in the China-Myanmar border region.

ABSTRACT:

Background

Mass screening and treatment (MSAT) for malaria elimination lacks an ideal diagnostic tool to allow sensitive and affordable test of the target population in the field. This study evaluated whether Capture and Ligation Probe-PCR (CLIP-PCR) could be used in a field MSAT in Laiza City, Myanmar.

Methods

On day 0, two dried blood spots were collected from each participant. On day 1, all samples were screened for Plasmodium in a 20 m² laboratory with workbench, a biosafety cabinet, a refrigerator, a benchtop shaking incubator and a qPCR machine, by four technicians using CLIP-PCR with sample pooling, at a health clinic of the Chinese bordering town of Nabang. On day 2, all positives were followed up and treated.

Results

Of 15,038 persons (65% of the t

SUBMITTER: Sun XD 

PROVIDER: S-EPMC9854064 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature