A Two-Compartment Fermentation System to Quantify Strain-Specific Interactions in Microbial Co-Cultures.

ABSTRACT: To fulfil the growing interest in investigating microbial interactions in co-cultures, a novel two-compartment bioreactor system was developed, characterised, and implemented. The system allowed for the exchange of amino acids and peptides via a polyethersulfone membrane that retained biomass. Further system characterisation revealed a Bodenstein number of 18, which hints at backmixing. Together with other physical settings, the existence of unwanted inner-compartment substrate gradients could be ruled out. Furthermore, the study of Damkoehler numbers indicated that a proper metabolite supply between compartments was enabled. Implementing the two-compartment system (2cs) for growing Streptococcus thermophilus and Lactobacillus delbrueckii subs. bulgaricus, which are mi

SUBMITTER: Ulmer A 

PROVIDER: S-EPMC9854596 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature