Critical Role of Cathepsin L/V in Regulating Endothelial Cell Senescence.

ABSTRACT: The senescence of vascular endothelial cells (ECs) is characterized as a hallmark of vascular aging, which leads to the initiation, progress, and advancement of cardiovascular diseases. However, the mechanism of the ECs senescence remains elusive. In this study, thoracic aortas were separated from young (8-week-old) and aged (18-month-old) mice. Decreased Ctsl expression and increased vascular remodeling were observed in senescent aorta. H₂O₂ was used to induce human umbilical vein endothelial cells (HUVECs) senescence, as shown by increased SA-β-gal positive cells and upregulated p21 level. CTSV significantly decreased after H₂O₂ treatment, while over-expression of CTSV by adenovirus reduced cellular senescence. RNA sequencing analysis was condu

SUBMITTER: Li C 

PROVIDER: S-EPMC9855167 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature