ETS1-HMGA2 Axis Promotes Human Limbal Epithelial Stem Cell Proliferation.

ABSTRACT:

Purpose

This study aimed to investigate the role and molecular mechanism of ETS1 in the proliferation and differentiation of human limbal epithelial stem cells (LESCs).

Methods

RNA-seq and quantitative real-time PCR were used to determine gene expression changes when ETS1 and HMGA2 was knocked down using short-hairpin RNAs or overexpressed by lentivirus. Immunofluorescence and flow cytometry experiments were performed to assess the roles of ETS1 and HMGA2 in LESC proliferation. ETS1-bound cis-regulatory elements and target genes in LESCs were identified using chromatin immunoprecipitation sequencing. The epigenetic features of ETS1-binding sites were assessed by the published histone modification and chromatin accessibility profiles.

Results

ETS1 was robustly expresse

SUBMITTER: Wang B 

PROVIDER: S-EPMC9855287 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature