Expression of a Salt-Tolerant Pseudolysin in Yeast for Efficient Protein Hydrolysis under High-Salt Conditions.

ABSTRACT: Protease biocatalysis in a high-salt environment is very attractive for applications in the detergent industry, the production of diagnostic kits, and traditional food fermentation. However, high-salt conditions can reduce protease activity or even inactivate enzymes. Herein, in order to explore new protease sources, we expressed a salt-tolerant pseudolysin of Pseudomonas aeruginosa SWJSS3 isolated from deep-sea mud in Saccharomyces cerevisiae. After optimizing the concentration of ion cofactors in yeast peptone dextrose (YPD) medium, the proteolytic activity in the supernatant was 2.41 times more than that in the control group when supplemented with 5 mM CaCl₂ and 0.4 mM ZnCl₂. The extracellular proteolytic activity of pseudolysin reached 258.95 U/mL wi

SUBMITTER: Liu X 

PROVIDER: S-EPMC9855795 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature