Modeling Human Lung Cells Exposure to Wildfire Uncovers Aberrant lncRNAs Signature.

ABSTRACT: Emissions generated by wildfires are a growing threat to human health and are characterized by a unique chemical composition that is tightly dependent on geographic factors such as fuel type. Long noncoding RNAs (lncRNAs) are a class of RNA molecules proven to be critical to many biological processes, and their condition-specific expression patterns are emerging as prominent prognostic and diagnostic biomarkers for human disease. We utilized a new air-liquid interface (ALI) direct exposure system that we designed and validated in house to expose immortalized human tracheobronchial epithelial cells (AALE) to two unique wildfire smokes representative of geographic regions (Sierra Forest and Great Basin). We conducted an RNAseq analysis on the exposed cell cultures and proved through both pri

SUBMITTER: Nguyen PK 

PROVIDER: S-EPMC9855943 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature