Single-Cell Transcriptome Analysis of H5N1-HA-Stimulated Alpaca PBMCs.

ABSTRACT: Avian influenza A virus H5N1 is a highly pathogenic and persistently a major threat to global health. Vaccines and antibodies targeting hemagglutinin (HA) protein are the primary management strategies for the epidemic virus. Although camelids possess unique immunological features, the immune response induced by specific antigens has not yet been thoroughly investigated. Herein, we immunized an alpaca with the HA antigen of the H5N1 virus and performed single-cell transcriptome profiling for analysis of longitudinal peripheral blood mononuclear cell (PBMCs) behavior using single-cell sequencing technology (scRNA-seq). We revealed multiple cellular immunities during the immunization. The monocytes continued to expand after immunization, while the plasma cells reached their peak three days af

SUBMITTER: Lyu M 

PROVIDER: S-EPMC9855979 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature