Intact Transition Epitope Mapping-Force Differences between Original and Unusual Residues (ITEM-FOUR).

ABSTRACT: Antibody-based point-of-care diagnostics have become indispensable for modern medicine. In-depth analysis of antibody recognition mechanisms is the key to tailoring the accuracy and precision of test results, which themselves are crucial for targeted and personalized therapy. A rapid and robust method is desired by which binding strengths between antigens and antibodies of concern can be fine-mapped with amino acid residue resolution to examine the assumedly serious effects of single amino acid polymorphisms on insufficiencies of antibody-based detection capabilities of, e.g., life-threatening conditions such as myocardial infarction. The experimental ITEM-FOUR approach makes use of modern mass spectrometry instrumentation to investigate intact immune complexes in the gas phase. ITEM-FOUR

SUBMITTER: Rower C 

PROVIDER: S-EPMC9856199 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature