Characterization of Hepatoma-Derived Growth Factor-Related Protein 2 Interactions with Heterochromatin.

ABSTRACT: The expression of genetic information is tightly controlled by chromatin regulatory proteins, including those in the heterochromatin gene repression family. Many of these regulatory proteins work together on the chromatin substrate to precisely regulate gene expression during mammalian development, giving rise to many different tissues in higher organisms from a fixed genomic template. Here we identify and characterize the interactions of two related heterochromatin regulatory proteins, heterochromatin protein 1 alpha (HP1α) and M-phase phosphoprotein 8 (MPP8), with hepatoma-derived growth factor-related protein 2 (HRP2). We find in biochemical experiments that HRP2 copurifies and co-sediments with heterochromatin-associated proteins, including HP1α and MPP8. Using the Chromatin in vivo As

SUBMITTER: Wistner SC 

PROVIDER: S-EPMC9856275 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature