Silencing of circCRIM1 Drives IGF2BP1-Mediated NSCLC Immune Evasion.

ABSTRACT:

Objectives

Circular RNAs (circRNAs) have been found to have significant impacts on non-small cell lung cancer (NSCLC) progression through various mechanisms. However, the mechanism of circRNAs modulating tumor immune evasion in NSCLC has yet to be well-revealed.

Materials and methods

Through analyzing the expression profiles of circRNAs in NSCLC tissues, RNA FISH, pull-down assay, mass spectrometry analysis, and RIP, circCRIM1 was identified, and its interaction with IGF2BP1 was confirmed. The effects of circCRIM1 on modulating tumor immune evasion were explored via co-culture in vitro and in tumor xenograft models. Subsequently, we evaluated the regulatory effects of circCRIM1 on IGF2BP1 and screened its target genes through RNA sequencing. Finally, we explored the underlyi

SUBMITTER: Peng W 

PROVIDER: S-EPMC9856323 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature