Multiplexed smFRET Nucleic Acid Sensing Using DNA Nanotweezers.

ABSTRACT: The multiplexed detection of disease biomarkers is part of an ongoing effort toward improving the quality of diagnostic testing, reducing the cost of analysis, and accelerating the treatment processes. Although significant efforts have been made to develop more sensitive and rapid multiplexed screening methods, such as microarrays and electrochemical sensors, their limitations include their intricate sensing designs and semi-quantitative detection capabilities. Alternatively, fluorescence resonance energy transfer (FRET)-based single-molecule counting offers great potential for both the sensitive and quantitative detection of various biomarkers. However, current FRET-based multiplexed sensing typically requires the use of multiple excitation sources and/or FRET pairs, which complicates lab

SUBMITTER: Kaur A 

PROVIDER: S-EPMC9856376 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature