Tumor-Educated Platelet Extracellular Vesicles: Proteomic Profiling and Crosstalk with Colorectal Cancer Cells.

ABSTRACT:

Background

Platelet-cancer cell interactions modulate tumor metastasis and thrombosis in cancer. Platelet-derived extracellular vesicles (EVs) can contribute to these outcomes.

Methods

We characterized the medium-sized EVs (mEVs) released by thrombin-stimulated platelets of colorectal cancer (CRC) patients and healthy subjects (HS) on the capacity to induce epithelial-mesenchymal transition (EMT)-related genes and cyclooxygenase (COX)-2(PTGS2), and thromboxane (TX)B₂ production in cocultures with four colorectal cancer cell lines. Platelet-derived mEVs were assessed for their size distribution and proteomics signature.

Results

The mEV population released from thrombin-activated platelets of CRC patients had a different size distribution vs. HS. Plate

SUBMITTER: Contursi A 

PROVIDER: S-EPMC9856452 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature