Triphasic 3D In Vitro Model of Bone-Tendon-Muscle Interfaces to Study Their Regeneration.

ABSTRACT: The transition areas between different tissues, known as tissue interfaces, have limited ability to regenerate after damage, which can lead to incomplete healing. Previous studies focussed on single interfaces, most commonly bone-tendon and bone-cartilage interfaces. Herein, we develop a 3D in vitro model to study the regeneration of the bone-tendon-muscle interface. The 3D model was prepared from collagen and agarose, with different concentrations of hydroxyapatite to graduate the tissues from bones to muscles, resulting in a stiffness gradient. This graduated structure was fabricated using indirect 3D printing to provide biologically relevant surface topographies. MG-63, human dermal fibroblasts, and Sket.4U cells were found suitable cell models for bones, tendons, and muscles, respectiv

SUBMITTER: Balestri W 

PROVIDER: S-EPMC9856925 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature