MiR-1246 is implicated as a possible candidate for endometrium remodelling facilitating implantation in buffalo (Bubalus bubalis).

ABSTRACT:

Background

The microRNAs (miRs) secreted by the trophectoderm (TE) cells have recently been implicated in the conceptus-endometrial cross talk during implantation and placentation. These miRs modulate various cellular processes during conception and throughout the pregnancy by regulating the gene expression in the foetal and maternal tissues.

Objectives

This study was undertaken to elucidate the function of TE secreted miRNAs in the maternal-foetal cross-talk during implantation/placentation in buffalo.

Methods

The in vitro produced blastocysts were cultured on a cumulus feeder layer for 21 days. The relative expression profiles of a selected panel of miRs was generated using the spent media collected on Days 0, 7, 12, 16, and 21. A custom-designed mirVana™ miRNA mimi

SUBMITTER: Dubey P 

PROVIDER: S-EPMC9857007 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature