A Novel Window into Angiogenesis-Intravital Microscopy in the AV-Loop-Model.

ABSTRACT: Due to the limitations of current in vivo experimental designs, our comprehensive knowledge of vascular development and its implications for the development of large-scale engineered tissue constructs is very limited. Therefore, the purpose of this study was to develop unique in vivo imaging chambers that allow the live visualization of cellular processes in the arteriovenous (AV) loop model in rats. We have developed two different types of chambers. Chamber A is installed in the skin using the purse sting fixing method, while chamber B is installed subcutaneously under the skin. Both chambers are filled with modified gelatin hydrogel as a matrix. Intravital microscopy (IVM) was performed after the injection of fluorescein isothiocyanate (FITC)-labeled dextran and rhodamine 6G dye. The AV

SUBMITTER: Vaghela R 

PROVIDER: S-EPMC9857023 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature