Characterization of pig skeletal muscle transcriptomes in response to low temperature.

ABSTRACT:

Objectives

The response of mammals to cold environment is a complex physiological activity, and its underlying mechanism must be analyzed from multiple perspectives. Skeletal muscle is an important thermogenic tissue that maintains body temperature in mammals. We dissected the molecular mechanism of pig skeletal muscle response to a cold environment by performing comparative transcriptome analysis in the Enshi black pig.

Methods

Three pigs were subjected to acute cold stress (3 days), three pigs were subjected to cold acclimation (58 days), and three pigs were used as controls. RNA-seq was used to screen the differentially expressed genes (DEGs) of skeletal muscle.

Results

Using RNA-seq methods, we identified 1241 DEGs within the acute cold stress group and 1886 DEGs

SUBMITTER: Zhang D 

PROVIDER: S-EPMC9857100 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature