TBX2 affects proliferation, apoptosis and cholesterol generation by regulating mitochondrial function and autophagy in bovine cumulus cell.

ABSTRACT:

Background

T-box transcription factor 2 (TBX2) is a member of T-box gene family whose members are highly conserved in evolution and encoding genes and are involved in the regulation of developmental processes. The encoding genes play an important role in growth and development. Although TBX2 has been widely studied in cancer cell growth and development, its biological functions in bovine cumulus cells remain unclear.

Objectives

This study aimed to investigate the regulatory effects of TBX2 in bovine cumulus cells.

Methods

TBX2 gene was knockdown with siRNA to clarify the function in cellular physiological processes. Cell proliferation and cycle changes were determined by xCELLigence cell function analyzer and flow cytometry. Mitochondrial membrane potential and autoph

SUBMITTER: Li SP 

PROVIDER: S-EPMC9857127 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature