Preparation, Identification and Application of β-Lactoglobulin Hydrolysates with Oral Immune Tolerance.

ABSTRACT: To reveal, for the first time, the mechanism of T cell epitope release from β-lactoglobulin that induces oral immune tolerance, a strategy for the prediction, preparation, identification and application of β-lactoglobulin hydrolysate with oral immune tolerance was established using the bioinformatics method, hydrolysis, mass spectrometry, T cell proliferation assays and animal experiments. Some T cell epitope peptides of β-lactoglobulin were identified for the first time. The hydrolysates of trypsin, protamex and papain showed oral tolerance, among which the hydrolysates of protamex and papain have been reported for the first time. Although the neutral protease hydrolysate contained T cell epitopes, it still had allergenicity. The mechanism behind oral immune tolerance induction by T cell

SUBMITTER: Tian L 

PROVIDER: S-EPMC9857568 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature