Flow Cytometry Detection of Anthracycline-Treated Breast Cancer Cells: An Optimized Protocol.

ABSTRACT: The use of anthracycline derivatives was approved for the treatment of a broad spectrum of human tumors (i.e., breast cancer). The need to test these drugs on cancer models has pushed the basic research to apply many types of in vitro assays, and, among them, the study of anthracycline-induced apoptosis was mainly based on the application of flow cytometry protocols. However, the chemical structure of anthracycline derivatives gives them a strong autofluorescence effect that must be considered when flow cytometry is used. Unfortunately, the guidelines on the analysis of anthracycline effects through flow cytometry are lacking. Therefore, in this study, we optimized the flow cytometry detection of doxorubicin and epirubicin-treated breast cancer cells. Their autofluorescence was assessed bo

SUBMITTER: Catitti G 

PROVIDER: S-EPMC9857732 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature