Downregulation of Peroxidase Activity of Platinum Cube Enables Minute-Time Scale Colorimetric Signaling of Hypoxanthine for Fish Freshness Monitoring.

ABSTRACT: Due to its unique biological composition, aquatic products, especially fish, are extremely perishable compared to other muscle products. Herein, we proposed an artificial nanozyme-based colorimetric detection of hypoxanthine (Hx), the indicator of fish freshness, in a minute-time scale without the assistance of a natural enzyme (hypoxanthine oxidase). The principle is based on the interaction between Hx and polyvinylpyrrolidone-modified platinum cubic nanomaterials (PVP-PtNC), in which the catalytic active sites of PVP-PtNC's surface were blocked by Hx. This causes the downregulation of PVP-PtNC's catalytic ability and weakened its ability to catalyze the oxidization of 3,3',5,5'-Tetramethylbenzidine (TMB) by H₂O₂. Accordingly, the decrease in the UV-vis absorption an

SUBMITTER: Ma X 

PROVIDER: S-EPMC9858192 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature