The Role of Pancreatic Fatty Acid Synthesis in Islet Morphology and Function after Caloric Restriction or Roux-En-Y Gastric Bypass Surgery in Mice.

ABSTRACT:

Background

Both caloric restriction (CR) and Roux-en-Y gastric bypass (RYGB) are practical interventions for type 2 diabetes mellitus (T2DM), while the molecular mechanisms of CR and RYGB regarding glycemic control are still poorly understood. Here, we explore the effects and underlying mechanisms of CR and RYGB on β-cell area and function.

Methods

Average islet size was measured by histological analysis. The pancreatic lipid content was detected by using a commercial lipid assay kit. The expression levels of lipogenic transcription factors and enzymes in mouse pancreas were determined by quantitative PCR, Western blot, and immunofluorescence.

Results

CR decreased the mean size of islets and pancreatic insulin production in both regular diet-fed and high-fat diet-fed

SUBMITTER: Mo H 

PROVIDER: S-EPMC9858859 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature