Interdependent progression of bidirectional sister replisomes in <i>E. coli</i>.

ABSTRACT: Bidirectional DNA replication complexes initiated from the same origin remain colocalized in a factory configuration for part or all their lifetimes. However, there is little evidence that sister replisomes are functionally interdependent, and the consequence of factory replication is unknown. Here, we investigated the functional relationship between sister replisomes in Escherichia coli, which naturally exhibits both factory and solitary configurations in the same replication cycle. Using an inducible transcription factor roadblocking system, we found that blocking one replisome caused a significant decrease in overall progression and velocity of the sister replisome. Remarkably, progression was impaired only if the block occurred while sister replisomes were still in a factory con

SUBMITTER: Chen PJ 

PROVIDER: S-EPMC9859026 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature