A new PEDV strain CH/HLJJS/2022 can challenge current detection methods and vaccines.

ABSTRACT:

Background

Porcine epidemic diarrhea virus (PEDV) variant strains cause great economic losses to the global swine industry. However, vaccines do not provide sufficient protection against currently circulating strains due to viral mutations. This study traced the molecular characteristics of the most recent isolates in China and aimed to provide a basis for the prevention and treatment of PEDV.

Methods

We obtained samples from a Chinese diarrheal swine farm in 2022. Reverse transcription polymerase chain reaction and immunofluorescence were used to determine the etiology, and the full-length PEDV genome was sequenced. Nucleotide similarity was calculated using MEGA to construct a phylogenetic tree and DNASTAR. Mutant amino acids were aligned using DNAMAN and modeled by SWISS-

SUBMITTER: Yao X 

PROVIDER: S-EPMC9859669 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature