Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids.

ABSTRACT:

Objectives

Primary cilia are conserved organelles found in polarized mammalian cells that regulate neuronal growth, migration, and differentiation. Proper cilia formation is essential during eye development. Our previous reports found that both amacrine and retinal ganglion cells (RGCs) contain primary cilia in primate and rodent retinas. However, whether primary cilia are present in the inner retina of human retinal organoids remains unknown. The purpose of this study is to characterize the primary cilia distribution in human embryonic stem cell (hESC-derived retinal organoid development.

Materials and methods

Retinal organoids were differentiated from a hESC line, harvested at various developmental timepoints (day 44-day 266), and immunostained with antibodies for primary

SUBMITTER: Ning K 

PROVIDER: S-EPMC9859708 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature