Tools to compare antibody gold nanoparticle conjugates for a small molecule immunoassay.

ABSTRACT: Antibody gold nanoparticle conjugates as recognition elements are essential for the overall performance of lateral flow assays. When immobilizing antibodies on gold nanoparticles, the challenge is to prevent aggregation and to ensure that the antibodies are correctly oriented so that they remain functional and their paratopes remain accessible. There are many methods available, and it is difficult to decide which one to use. To help selecting the most appropriate conjugate production method, different synthetic routes of binding antibodies to gold nanoparticles are systematically investigated for the purpose of a quantitative lateral flow test for small molecules. The direct comparison of different conjugate syntheses shows how to select a suitable conjugate for a lateral flow assay. The s

SUBMITTER: Conrad M 

PROVIDER: S-EPMC9859872 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature