Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiation.

ABSTRACT: Vascular endothelial cells are a mesoderm-derived lineage with many essential functions, including angiogenesis and coagulation. The gene-regulatory mechanisms underpinning endothelial specialization are largely unknown, as are the roles of chromatin organization in regulating endothelial cell transcription. To investigate the relationships between chromatin organization and gene expression, we induced endothelial cell differentiation from human pluripotent stem cells and performed Hi-C and RNA-sequencing assays at specific time points. Long-range intrachromosomal contacts increase over the course of differentiation, accompanied by widespread heteroeuchromatic compartment transitions that are tightly associated with transcription. Dynamic topologically associating domain boundaries strengt

SUBMITTER: Alavattam KG 

PROVIDER: S-EPMC9860068 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature