Tandem mass tag-based thermal proteome profiling for the discovery of drug-protein interactions in cancer cells.

ABSTRACT: Identification of effector targets is imperative to the characterization of the mechanisms of action of novel small molecules. Here, we describe steps to identify effector drug-protein interactions in lysates derived from cancer cell lines using a thermal proteome profiling (TPP) protocol. Building on existing TTP approaches, we detail the use of an in-solution trypsin digestion technique to streamline sample preparation, a nonparametric analysis to rank proteins for prioritization, and a follow-up strategy for identifying effector interactors. For complete details on the use and execution of this protocol, please refer to Johnson et al. (2022).¹.

SUBMITTER: Johnson FD 

PROVIDER: S-EPMC9860163 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature