Absolute quantification of viable bacteria abundances in food by next-generation sequencing: Quantitative NGS of viable microbes.

ABSTRACT: Next-generation sequencing (NGS) is an important tool for taxonomical bacteria identification. Recent technological developments have led to its improvement and availability. Despite the undeniable advantages of this approach, it has several limitations and shortcomings. The usual outcome of microbiota sequencing is a relative abundance of bacterial taxa. The information about bacteria viability or enumeration is missing. However, this knowledge is crucial for many applications. In the current study, we elaborated the complete workflow for the absolute quantification of living bacteria based on 16S rRNA gene amplicon sequencing. A fluorescent PMAxx reagent penetrating a damaged cell membrane was used to discriminate between the total and viable bacterial population. Bacteria enumeration wa

SUBMITTER: Kallastu A 

PROVIDER: S-EPMC9860258 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature