Regulation of EGF-stimulated activation of the PI-3K/AKT pathway by exocyst-mediated exocytosis.

ABSTRACT: The phosphoinositide-3 kinase (PI-3K)/AKT cell survival pathway is an important pathway activated by EGFR signaling. Here we show, that in addition to previously described critical components of this pathway, i.e., the docking protein Gab1, the PI-3K/AKT pathway in epithelial cells is regulated by the exocyst complex, which is a vesicle tether that is essential for exocytosis. Using live-cell imaging, we demonstrate that PI(3,4,5)P₃ levels fluctuate at the membrane on a minutes time scale and that these fluctuations are associated with local PI(3,4,5)P₃ increases at sites where recycling vesicles undergo exocytic fusion. Supporting a role for exocytosis in PI(3,4,5)P₃ generation, acute promotion of exocytosis by optogenetically driving exocyst-mediated vesi

SUBMITTER: An SJ 

PROVIDER: S-EPMC9860279 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature