Effects of sample matrix in the measurement of antithrombin by LC-MS: A role for immunocapture.

ABSTRACT:

Introduction

The sample matrix composition, which is greatly affected by the type of blood collection tube used during phlebotomy, is of major importance in laboratory testing as it can influence test results. We developed an LC-MRM-MS test to molecularly characterize antithrombin in citrate plasma. The test principle differs greatly from traditional laboratory tests and the influence of varying plasma sample matrices is largely unknown.

Objectives

To identify whether variations in sample matrix affect the LC-MRM-MS test for antithrombin and assess whether sample pre-processing by immunocapture reduces matrix-specific effects.

Methods

Samples (n = 45) originating from four different blood collection tubes (sodium citrate, lithium heparin, K₂-EDTA and K

SUBMITTER: Kruijt M 

PROVIDER: S-EPMC9860377 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature