Closed-Loop Microreactor on PCB for Ultra-Fast DNA Amplification: Design and Thermal Validation.

ABSTRACT: Polymerase chain reaction (PCR) is the most common method used for nucleic acid (DNA) amplification. The development of PCR-performing microfluidic reactors (μPCRs) has been of major importance, due to their crucial role in pathogen detection applications in medical diagnostics. Closed loop (CL) is an advantageous type of μPCR, which uses a circular microchannel, thus allowing the DNA sample to pass consecutively through the different temperature zones, in order to accomplish a PCR cycle. CL μPCR offers the main advantages of the traditional continuous-flow μPCR, eliminating at the same time most of the disadvantages associated with the long serpentine microchannel. In this work, the performance of three different CL μPCRs designed for fabrication on a printed circuit board (PCB) was evalu

SUBMITTER: Skaltsounis P 

PROVIDER: S-EPMC9860919 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature