Identification and Molecular Characterization of Four New Blastocystis Subtypes Designated ST35-ST38.
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ABSTRACT:
SUBMITTER: Maloney JG
PROVIDER: S-EPMC9860945 | biostudies-literature | 2022 Dec
REPOSITORIES: biostudies-literature
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Identification and Molecular Characterization of Four New <i>Blastocystis</i> Subtypes Designated ST35-ST38.
Maloney Jenny G JG Molokin Aleksey A Seguí Raimundo R Maravilla Pablo P Martínez-Hernández Fernando F Villalobos Guiehdani G Tsaousis Anastasios D AD Gentekaki Eleni E Muñoz-Antolí Carla C Klisiowicz Debora R DR Oishi Camila Y CY Toledo Rafael R Esteban J Guillermo JG Köster Pamela C PC de Lucio Aida A Dashti Alejandro A Bailo Begoña B Calero-Bernal Rafael R González-Barrio David D Carmena David D Santín Mónica M
Microorganisms 20221223 1
Three recent studies of <i>Blastocystis</i> epidemiology in mammalian hosts identified four novel sequences that appeared to share <i>B. lapemi</i> as the most similar sequence. However, full-length <i>ssu</i> rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were ...[more]
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Project description:Blastocystis is an anaerobic unicellular protozoal parasite infecting the gastrointestinal tract of humans and a wide range of animals. It is one of the most common enteric microorganisms with higher prevalence rates in developing than in developed countries. Feco-oral is the main route of transmission where low socioeconomic conditions, poor hygienic practices, close contact with animals, and drinking contaminated water act as major risk factors. Infection with Blastocystis was demonstrated in both symptomatic and asymptomatic people. For a long period, Blastocystis was considered a commensal organism with no pathogenic role, but recently, many studies linked it to different gastrointestinal symptoms such as nausea, diarrhea, and abdominal pain. Association with irritable bowel syndrome and colorectal cancer was also reported.
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