Towards the Laboratory Maintenance of Haemagogus janthinomys (Dyar, 1921), the Major Neotropical Vector of Sylvatic Yellow Fever.

ABSTRACT: Haemagogus (Haemagogus) janthinomys (Dyar, 1921), the major neotropical vector of sylvatic yellow fever virus, is notoriously difficult to maintain in captivity. It has never been reared beyond an F₁ generation, and almost no experimental transmission studies have been performed with this species since the 1940s. Herein we describe installment hatching, artificial blood feeding, and forced-mating techniques that enabled us to produce small numbers of F₃ generation Hg. janthinomys eggs for the first time. A total of 62.8% (1562/2486) F₁ generation eggs hatched during ≤10 four-day cycles of immersion in a bamboo leaf infusion followed by partial drying. Hatching decreased to 20.1% (190/944) in the F₂ generation for eggs laid by mosqui

SUBMITTER: Hendy A 

PROVIDER: S-EPMC9861039 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature