Mass Spectrometric Blood Metabogram: Acquisition, Characterization, and Prospects for Application.

ABSTRACT: In metabolomics, many metabolites are measured simultaneously in a single run. Such analytical performance opens up prospects for clinical laboratory diagnostics. In this work, a mass spectrometric metabogram was developed as a simplified and clinically applicable way of measuring the blood plasma metabolome. To develop the metabogram, blood plasma samples from healthy male volunteers (n = 48) of approximately the same age, direct infusion mass spectrometry (DIMS) of the low molecular fraction of samples, and principal component analysis (PCA) of the mass spectra were used. The seven components of the metabogram defined by PCA, which cover ~70% of blood plasma metabolome variability, were characterized using a metabolite set enrichment analysis (MSEA) and clinical test results of participa

SUBMITTER: Lokhov PG 

PROVIDER: S-EPMC9861083 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature