Dental Pulp Inflammation Initiates the Occurrence of Mast Cells Expressing the α₁ and β₁ Subunits of Soluble Guanylyl Cyclase.

ABSTRACT: The binding of nitric oxide (NO) to heme in the β₁ subunit of soluble guanylyl cyclase (sGC) activates both the heterodimeric α₁β₁ and α₂β₁ isoforms of the enzyme, leading to the increased production of cGMP from GTP. In cultured human mast cells, exogenous NO is able to inhibit mast cell degranulation via NO-cGMP signaling. However, under inflammatory oxidative or nitrosative stress, sGC becomes insensitive to NO. The occurrence of mast cells in healthy and inflamed human tissues and the in vivo expression of the α₁ and β₁ subunits of sGC in human mast cells during inflammation remain largely unresolved and were investigated here. Using peroxidase and double immunohistochemical incubations, no mast cells were fou

SUBMITTER: Korkmaz Y 

PROVIDER: S-EPMC9861465 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature