The Anaesthetics Isoflurane and Xenon Reverse the Synaptotoxic Effects of Aβ_1-42 on Megf10-Dependent Astrocytic Synapse Elimination and Spine Density in Ex Vivo Hippocampal Brain Slices.

ABSTRACT: It has been hypothesised that inhalational anaesthetics such as isoflurane (Iso) may trigger the pathogenesis of Alzheimer's disease (AD), while the gaseous anaesthetic xenon (Xe) exhibits many features of a putative neuroprotective agent. Loss of synapses is regarded as one key cause of dementia in AD. Multiple EGF-like domains 10 (MEGF10) is one of the phagocytic receptors which assists the elimination of synapses by astrocytes. Here, we investigated how β-amyloid peptide 1-42 (Aβ_1-42), Iso and Xe interact with MEGF10-dependent synapse elimination. Murine cultured astrocytes as well as cortical and hippocampal ex vivo brain slices were treated with either Aβ_1-42, Iso or Xe and the combination of Aβ_1-42 with either Iso or Xe. We quantified MEGF10 expressio

SUBMITTER: Shi D 

PROVIDER: S-EPMC9861496 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature