Functionalized Magnetite Nanoparticles: Characterization, Bioeffects, and Role of Reactive Oxygen Species in Unicellular and Enzymatic Systems.

ABSTRACT: The current study evaluates the role of reactive oxygen species (ROS) in bioeffects of magnetite nanoparticles (MNPs), such as bare (Fe₃O₄), humic acids (Fe₃O₄-HA), and 3-aminopropyltriethoxysilane (Fe₃O₄-APTES) modified MNPs. Mössbauer spectroscopy was used to identify the local surrounding for Fe atom/ions and the depth of modification for MNPs. It was found that the Fe₃O₄-HA MNPs contain the smallest, whereas the Fe₃O₄-APTES MNPs contain the largest amount of Fe²⁺ ions. Bioluminescent cellular and enzymatic assays were applied to monitor the toxicity and anti-(pro-)oxidant activity of MNPs. The contents of ROS were determined by a chemiluminescence luminol assay evalu

SUBMITTER: Kicheeva AG 

PROVIDER: S-EPMC9861541 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature