The ERAD Pathway Participates in Fungal Growth and Cellulase Secretion in Trichoderma reesei.

ABSTRACT: Trichoderma reesei is a powerful fungal cell factory for the production of cellulolytic enzymes due to its outstanding protein secretion capacity. Endoplasmic reticulum-associated degradation (ERAD) plays an integral role in protein secretion that responds to secretion pressure and removes misfolded proteins. However, the role of ERAD in fungal growth and endogenous protein secretion, particularly cellulase secretion, remains poorly understood in T. reesei. Here, we investigated the ability of T. reesei to grow under different stresses and to secrete cellulases by disrupting three major genes (hrd1, hrd3 and der1) involved in the critical parts of the ERAD pathway. Under the ER stress induced by high concentrations of DTT, knockout of hrd1,

SUBMITTER: Yao C 

PROVIDER: S-EPMC9862206 | biostudies-literature | 2023 Jan

REPOSITORIES: biostudies-literature