Systematic comparison of transcriptomes of Caco-2 cells cultured under different cellular and physiological conditions.

ABSTRACT: There is a need for standardized in vitro models emulating the functionalities of the human intestinal tract to study human intestinal health without the use of laboratory animals. The Caco-2 cell line is a well-accepted and highly characterized intestinal barrier model, which has been intensively used to study intestinal (drug) transport, host-microbe interactions and chemical or drug toxicity. This cell line has been cultured in different in vitro models, ranging from simple static to complex dynamic microfluidic models. We aimed to investigate the effect of these different in vitro experimental variables on gene expression. To this end, we systematically collected and extracted data from studies in which transcriptome analyses were performed on Caco-2 cells grown on permeable membranes.

SUBMITTER: Elzinga J 

PROVIDER: S-EPMC9862247 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature